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High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material.

George N Bowers Jr · Robert B. Mccomb · Richard G. Christensen · Robert Schaffer

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Scientific Measurement and Uncertainty Evaluation Analytical Methods in Pharmaceuticals Clinical Laboratory Practices and Quality Control

Abstract

We describe specifications for high-purity 4-nitrophenol, which is suitable for spectrophotometric standardization. Such a reference material is needed in clinical enzymology to establish the proper molar absorptivity of 4-nitrophenol under final reaction conditions, particularly for measuring alkaline phosphatase activity in human serum. Some lots of 4-nitrophenol available commercially met these specifications, but several did not. The latter can be purified to meet our specifications by recrystallization or sublimation. The molar absorptivity of 4-nitrophenol (35 mumol/L) IN 10 mmol/L NaOH at 25 degrees C at 401 nm is 18380 +/- 90 L.mol-1.cm-1.

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