Vasopressin Biosynthesis. III.In VitroStudies

Abstract

Under appropriate conditions, the hypothalamo-neurohypophysial complex, removed intact from guinea pigs, incorporated 36S-cysteine or 3H-tyrosine into vasopressin and tissue proteins. The appearance of labeled hormone was time-dependent, required an energy source and was inhibited by puromycin. Whereas slices of hypothalamicmedian eminence (HME) tissue could also carry out the incorporation of isotopic amino acids into vasopressin in vitro, the distal portion of the neuron (infundibular stem and process) could not do so. As in the case of in vivo isotope experiments, the appearance of labeled hormone in vitro was preceded by a lag period. This lag period appears to be a unique characteristic of vasopressin biosynthesis. Slices of HME taken from guinea pigs deprived of water for 4 days prior to sacrifice incorporated 2–5 times more label into vasopressin than slices of HME taken from animals which had free access to water. (Endocrinology75: 934, 1964)

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