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Abstract Richard Bradley proposed that biological agents caused infectious disease. He agreed that air could transmit disease, but it had to contain a living agent, either visible or microscopic. To dismiss the idea that air alone could cause disease he included evidence provided by Robert Balle about a toxic grotto near Lake Averna, close to Naples, whose fumes were lethal to animals and humans yet that lethality was not contagious and occurred quickly, not a characteristic of ‘pestilential fevers’. In this grotto the surface water was covered with a ‘green scum’, a layer of green sulphur photosynthetic bacteria that could live in that anoxic environment, which contained hydrogen sulphide and carbon dioxide gas and was accessible to visible light. This environment was a natural enrichment culture for these bacteria. It was the first time that the presence of these bacteria was disclosed in the scientific literature.
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