Articlegreen2009

Diagnostic morphometric applicability of confocal laser scanning microscopy in Osteoarchaeology

Christina Papageorgopoulou · Gisela Anna Kuhn · Urs Ziegler · Frank Jakobus Rühli

10 citations0/0 files storedInternational Journal of Osteoarchaeology

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Forensic Anthropology and Bioarchaeology Studies Cultural Heritage Materials Analysis Paleopathology and ancient diseases

Abstract

Abstract The confocal laser scanning microscope (CLSM) has become an essential tool for a wide range of biological and clinical studies and imaging applications. The major imaging modes of the CLSM include single and serial optical sections from thick specimens (100 µm), multiple wavelength images and three‐dimensional reconstruction. Such images provide measurement of length, surface or volume of objects. Surprisingly, this advanced microscopic method has been very rarely used in anthropological research, although numerous clinical and basic science research projects showed its value to study, bone growth, bone micro‐architecture and 3D bone morphometry. We present the basic principles and advantages of CLSM and outline practical aspects of specimen preparation, image collection and digital image processing specifically appropriate for historical bone and hair material. Copyright © 2009 John Wiley & Sons, Ltd.

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2005Dental microwear texture analysis shows within-species diet variability in fossil hominins 1995Activity‐induced musculoskeletal stress markers (MSM) and subsistence strategy changes among ancient Hudson Bay Eskimos 1981Microscopical Focal Destruction (Tunnels) in Exhumed Human Bones 2001Paleohistopathology of bone: A new approach to the study of ancient diseases 2001Paleohistopathology of bone: A new approach to the study of ancient diseases 1992Histological age estimation using rib and clavicle 2005Tracing prehistoric activities: Musculoskeletal stress marker analysis of a stone-age population on the Island of Gotland in the Baltic sea 2007Muscle markers revisited: Activity pattern reconstruction with controls in a central California Amerind population

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