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Experimental paleoparasitology: identification of Trypanosoma cruzi DNA in desiccated mouse tissue.

Bastos Om · Amanda Brenda de Souza e Silva de Araújo · Ferreira Lf · Patrick Wincker · Morel Cm

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Paleopathology and ancient diseases Trypanosoma species research and implications Forensic Entomology and Diptera Studies

Abstract

The polymerase chain reaction (PCR) has been used for clinical diagnosis of infectious disease and to research ancient animal and microbiological DNA from a wide range of tissues. PCR was used to study the possibility of Trypanosoma cruzi kinetoplast DNA (kDNA) extraction from experimentally desiccated mouse tissue (heart, skeletal muscle, liver, spleen, and pancreas). The results obtained suggest the application of this technique to T. cruzi detection in archaeological material.

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2011Chagas disease in prehistory 2007Parasite findings in archeological remains: Diagnosis and interpretation 2014Review of the rodent paleoparasitological knowledge from South America 2016The Paleoparasitology in Brazil and Findings in Human Remains from South America: A Review

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