Collection of amino acids and DNA from fingerprints using hydrogels

Ward van Helmond · Vincent O’Brien · Robin de Jong · Jan H. van Esch · Sander Oldenhof · Marcel de Puit

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Forensic Fingerprint Detection Methods Forensic and Genetic Research Biometric Identification and Security

Abstract

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualisation at a later stage using the standard method of cyanoacrylation. We will demonstrate this method to also be capable of collecting DNA from fingerprints with a 20-60% yield in comparison to using a conventional cotton swab.

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References

2012Composition of fingermark residue: A qualitative and quantitative review 2004Changes in the lipid composition of latent fingerprint residue with time after deposition on a surface 2007Identification of Oxidation Products of Squalene in Solution and in Latent Fingerprints by ESI-MS and LC/APCI-MS 2015Forensic Identification of Gender from Fingerprints 2013LCMS Analysis of Fingerprints, the Amino Acid Profile of 20 Donors 1998Evaluation of Aspartic Acid Racemization Ratios in the Human Femur for Age Estimation 2010Consistency and reproducibility in fingermark deposition 2012Analysis of amino acids in latent fingerprint residue by capillary electrophoresis‐mass spectrometry

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