Effects of fingerprint development reagents on subsequent DNA analysis

Haneul Lee · Jihye Yim · Yong‐Bin Eom

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Forensic Fingerprint Detection Methods Forensic and Genetic Research Molecular Biology Techniques and Applications

Abstract

A variety of evidences are found at crime scenes. Fingerprint and DNA evidences are especially important in the process of identifying personal sources. Among evidences found at crime scenes, cigarette butts are important because they might contain both fingerprints and DNA. In this study, latent fingerprints were detected in cigarette butts using 1,8-diazafluoren-9-one (DFO) and 1,2-Indanedione/zinc chloride (1,2-IND/Zn). Next, DNA extraction and real-time qPCR were performed to quantify and identify the DNA present. Short tandem repeat (STR) profiling was also performed. The results showed that the quantity of DNA recovered was decreased by 16% in DFO-treated cigarettes and by 27% in 1,2-IND/Zn-treated cigarettes when compared to untreated controls. When the STR profiling results were compared with those of the control sample, DFO, and 1,2-IND/Zn reagent-treated DNA samples showed individualized genotyping at several loci. Results of this study showed that when cigarette butts were found, DFO and 1,2-IND/Zn reagents could be used for DNA profiling after fingerprint identification. However, the effect of DFO on STR profiling was less than that of 1,2-IND/Zn. Therefore, we recommend the use of DFO for fingerprinting cigarette butts if further DNA processing is planned.

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References

2004Fingerprints and Other Ridge Skin Impressions 2012Advances in Fingerprint Analysis 2012The Fingerprint Sourcebook 2010Initial Results on the Composition of Fingerprints and its Evolution as a Function of Time by GC/MS Analysis 1990The Use of 1,8-Diazafluoren-9-one (DFO) for the Fluorescent Detection of Latent Fingerprints on Paper. A Preliminary Evaluation 2006Optimisation and evaluation of 1,2-indanedione for use as a fingermark reagent and its application to real samples 2003Forensic Applications of Chemical Imaging: Latent Fingerprint Detection Using Visible Absorption and Luminescence 2011Effect of common fingerprint detection techniques on subsequent STR profiling

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2023Quantifying DNA loss in laboratory-created latent prints due to fingerprint processing 2021DNA recovery after sequential processing of latent fingerprints on copy paper

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