Comparison of Crime-lite® 82S, Polilight® PL400 and Polilight® PL500 for the detection of semen and saliva stains

Jasmine W. Tay · Jessica Joudo · Teresa Tran · Hoan Ta · Jamie L. Botting · Yoon C. Liew +2 more

13 citations0/0 files storedAustralian Journal of Forensic Sciences

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Forensic Fingerprint Detection Methods Forensic and Genetic Research Forensic Entomology and Diptera Studies

Abstract

Alternative light sources (ALS) are widely employed in forensic investigations for the identification of biological fluids, such as saliva and semen. While metal halide arc lamps are a common light source for many ALS, high intensity light-emitting diode (LED) globes are a more energy efficient alternative. To date, ALS operating with different light sources have not been evaluated against each other, and this study is the first to complete a side-by-side comparison of the arc lamps, Polilight® PL400 (PL400), Polilight® PL500 (PL500) and the LED-based Crime-lite® 82S (blue) (CL82S) for the detection of semen and saliva dilutions on various fabric materials. The absence or presence of fluorescence were scored independently by four experienced item examination scientists to reduce bias. The number of fluorescence stains detected using the CL82S (244 observations) was higher and brighter in intensity compared to those visualized using PL400 and PL500 (183 and 202 observations, respectively). Notably, dilutions of saliva which were not visible under PL400 and PL500 on a range of fabrics were detected under CL82S, which was statistically significant (p < 0.003). Taken together, CL82S is a better screening tool than the PL400 and PL500 for detection of dilute semen and saliva stains on various fabric types.

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References

2006The Use of Polilight ® in the Detection of Seminal Fluid, Saliva, and Bloodstains and Comparison with Conventional Chemical‐Based Screening Tests 1991Detection of semen and blood stains using polilight as a light source 2002An Alternate Light Source to Detect Semen 2000A rapid method to detect dried saliva stains swabbed from human skin using fluorescence spectroscopy 2006The use of an alternative light source to detect semen in clinical forensic medical practice 2015Searching for biological traces on different materials using a forensic light source and infrared photography 2011A rapid and noninvasive method to detect dried saliva stains from human skin using fluorescent spectroscopy 2017The adaptation of a 360° camera utilising an alternate light source (ALS) for the detection of biological fluids at crime scenes

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