Articlegreen1970

The isolation of three neurophysins from porcine posterior pituitary lobes

104 citations0/0 files storedBiochemical Journal

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Neuroendocrine regulation and behavior Neuropeptides and Animal Physiology Cardiovascular, Neuropeptides, and Oxidative Stress Research

Abstract

1. Three neurophysins, proteins that bind the polypeptide hormones oxytocin and vasopressin, have been isolated from acetone-dried porcine posterior pituitary lobes. The proteins have been named porcine neurophysins-I, -II and -III in order of their electrophoretic mobilities at pH8.1. 2. Electrophoretic comparison of the purified proteins, which are homogeneous on starch-gel electrophoresis, with the soluble proteins of fresh porcine posterior pituitary lobes extracted in 0.1m-HCl and in buffer pH8.1 suggests that the isolated proteins are native to the fresh tissue. 3. Neurophysins-I and -II are present in similar amounts in the tissue, whereas neurophysin-III is present only in small quantities. Acetone-dried tissue also contains traces of other hormone-binding neurophysin components. 4. All the neurophysins can bind both oxytocin and [8-lysine]-vasopressin. 5. The apparent molecular weights of the neurophysins increase with increasing protein concentration as measured by equilibrium sedimentation in the ultracentrifuge. 6. Neurophysins-I and -III are of similar molecular dimensions, contain one residue of methionine per molecule and lack histidine. The minimum molecular weight of neurophysin-I obtained by amino acid analysis is 9360. Neurophysin-II is of larger molecular dimensions than neurophysins-I and -III and can be separated from these by gel filtration on Sephadex G-75. It contains no histidine or methionine, and its minimum molecular weight has been estimated as 14020 by amino acid analysis. 7. Each of the three neurophysins possesses N-terminal alanine. 8. The possible biological significance of the existence of several neurophysins within one species is discussed.

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References

1960EFFECT OF MAGNESIUM ION ON THE RESPONSE OF THE RAT UTERUS TO NEUROHYPOPHYSIAL HORMONES AND ANALOGUES1 1961Starch-gel Electrophoresis of Anterior Pituitary Hormones 1964Evidence for a Precursor in Vasopressin Biosynthesis 1968The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and -II as complexes with [8-arginine]-vasopressin

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1971Tentative identification of a vasopressin–neurophysin and an oxytocin–neurophysin in the rat 1975THE ROLE OF NEUROPHYSIN PROTEINS: SUGGESTIONS FROM THE STUDY OF THEIR TRANSPORT AND TURNOVER*1971Localization of neurophysin-II in the hypothalamo-neurohypophysical system of the pig by immunofluorescence histochemistry 1971Amino Acid Sequence of Porcine Neurophysin-I 1973Activity of magnocellular neuroendocrine cells in the hypothalamus of unanaesthetized monkeys 1973Subcellular organization of neurophysins, oxytocin, [8−lysine]-vasopressin and adenosine triphosphatase in porcine posterior pituitary lobes

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