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The motivation theories play an important role in forming the human resource management system. Especially in modern organizations, Herzberg's two-factor theory of motivation can be used to study and create a motivation system. This article analyzes the relationship between the descriptive characteristics of respondents and hygienic / motivating factors, as well as the influence of these factors on career management of university staff and lecturers. To assess the respondent's own opinion during data collection, it was used a method of questionnaire, compiled using a 5-point Likert scale. The survey results were coded and used in the SPSS Statistics program. Factor analysis, one-way ANOVA-test analysis and regression analysis were carried out. This article reveals the influence of hygienic and motivating factors on career management in relation to employees and lecturers. That is, in the case of an increase the hygiene factor coefficient by one unit, the level of career management will decrease by 18.5%. On the contrary, there is a positive correlation between the motivating factor and career development. That is, if the motivating factor increases by one unit, the career development factor will increase by 43.5%. As a result of the study within the framework of the two-factor theory, it was found that both factors are important for the career development of staff and lecturers. However, motivating factors must be given more importance in order to achieve a positive result in career management. When motivation system is increased through fair and effective motivation practice, the desire of personnel to develop also increases.
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