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The purpose of this study is to explore gender differences and the mediating effect of self-regulation in the relationship between university students' information literacy and academic achievement in online learning. SEM and multi-group analysis were used by university students’ responses from 2020(12th) of the Korean Education Longitudinal Study 2005(KELS 2005). The study found that university students' information literacy directly affects academic achievement, and the mediating effect of self-regulation was significant between information literacy and academic achievement. In addition, the effect of information literacy on self-regulation was found to be significant in both males and females. This means that information literacy has a positively significant effect on self-regulation regardless of gender. Finally, it was confirmed that there was no difference between genders in the mediating effect of self-regulation, but the effect of self-regulation on academic achievement was higher in women. The results of this study suggest to accomplish academic achievement in online learning, it is necessary to strengthen self-regulation through the improvement of learners' information literacy, and in particular, continuous education and support are required to cultivate information literacy.
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