Fluorescence of Organic Binders in Painting Cross-sections

Donata Magrini · Susanna Bracci · Irina Crina Anca Sandu

25 citations0/0 files storedProcedia Chemistry

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Cultural Heritage Materials Analysis Conservation Techniques and Studies Building materials and conservation

Abstract

This research is focused on the application of immuno-fluorescence microscopy (IFM) to localize and identify proteinaceous materials in painting cross-sections. To find the best analytical protocol and the best fluorochrome, a comparison between two different fluorescent markers for the secondary antibody has been performed. An already known fluorochrome has been compared with a marker not commonly used to characterize painting materials. Parallel to IFM, staining tests by means of fluorescent selective reagents, have been performed. The study was carried out on standard and historical samples. Drawbacks and limits of the methods are here presented and solutions to overcome the encountered problems are discussed.

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References

2006The Use of Immunofluorescence Microscopy and Enzyme-Linked Immunosorbent Assayas Complementary Techniques for Protein Identification in Artists' Materials 2012Cross-Section and Staining-Based Techniques for Investigating Organic Materials in Painted and Polychrome Works of Art: A Review 2008Identification of proteins in painting cross-sections by immunofluorescence microscopy

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2021A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings 2016Immunochemical Methods Applied to Art-Historical Materials: Identification and Localization of Proteins by ELISA and IFM 2016Circularly polarized luminescence reveals interaction between commercial stains and protein matrices used in paintings 2018Method development for binding media analysis in painting cross‐sections by desorption electrospray ionization mass spectrometry

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