Article2001

Characterization of Proteins in Paint Media by Immunofluorescence. A Note on Methodological Aspects

Blanca Ramírez Barat · Sonsoles de la Viña

38 citations0/0 files storedStudies in Conservation

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Cultural Heritage Materials Analysis Advanced Proteomics Techniques and Applications Conservation Techniques and Studies

Abstract

A protocol for the characterization of proteins in paint media by immunofluorescent labelled antibodies is proposed. The main difficulties of the technique and their possible solutions are discussed. Positive results on egg white, in model samples, are reported.

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References

1971METHODS USED FOR THE IDENTIFICATION OF BINDING MEDIA IN ITALIAN PAINTINGS OF THE FIFTEENTH AND SIXTEENTH CENTURIES 1992Reliable identification of human albumin in ancient bone using ELISA and monoclonal antibodies 1989Detection of Ovalbumin in Paint Media by Immunofluorescence

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2012Single and multiplexed immunoassays for the chemiluminescent imaging detection of animal glues in historical paint cross-sections 2013A simple and reliable methodology to detect egg white in art samples 2015Influence of pigments and protein aging on protein identification in historically representative casein-based paints using enzyme-linked immunosorbent assay 2013Peptide mass mapping as an effective tool for historical mortar analysis 2012Analysis of egg-based model wall paintings by use of an innovative combined dot-ELISA and UPLC-based approach 2008Comparison between traditional strategies and classification technique (SIMCA) in the identification of old proteinaceous binders 2009Identification of protein binders in works of art by high-performance liquid chromatography–diode array detector analysis of their tryptic digests 2003Old master paintings – A fruitful field of activity for analysts: Targets, methods, outlook

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