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Mother-infant separation from the maternal perspective is the focus of 2 studies reported here. First, a questionnaire, the Maternal Separation Anxiety Scale (MSAS), was developed and administered to 620 mothers soon after their infants were born and again 3 months later. In study 2, maternal separation anxiety was assessed using the MSAS and other methods: an interview, an emotional status index (taken at the point of actual separation), and an observational index based on mothers' behavior during departure and reunion from their infants in a structured laboratory setting. Data from this multiple-measures approach supported the validity of the MSAS and verified the strength of the construct
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