Galileo goes to jail: and other myths about science and religion

Abstract

Gene products from recombinant plasmids containing pBR322 and phenotypically cryptic plasmids from Neisseria gonorrhoeae (poster presentation)Two phenotypically cryptic plasmids (p31788K and pG5449K) with slightly different restriction endonuclease patterns, from two different strains of N .gonorrhoeae, have been integrated in the Hind111 site of pBR322 and transformed into the minicell strain E. coli DS410.The protein profile of two ApTcS-transformants with recombinant plasmid (pTA9, pTA11) containing p31788K, and one transformant with a plasmid (pTBe) containing pG5449K, have been investigated by the minicell method.p31788K and pG5449K are integrated in the same orientation in pBR322.In addition to pBR322-coded proteins (mature and premature betalactamase), the strains with the recombinant plasmids all express proteins of 25,000 and 10,020 dalton.The strain with pTBe expresses two additional proteins of 22,300 and 10,070 dalton.It is reasonable to suppose that the difference in protein profile reflects differences in DNA sequence between the two gonococcal plasmids.

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