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Purpose – This study aims to analyze the perception of generations (Baby boomers, X and Y) about the influence of Holistic Helixes of Innovation on Eco-innovation, as well as Eco-innovation on Environmental Practices, Cleaner Production, Social Actions, Regional Development, Smart Cities and Sustainable Development. Theoretical framework – Holistic Helixes of Innovation, Eco-innovation, Environmental Practices, Cleaner Production, Social Actions, Regional Development, Smart Cities and Sustainable Development. Design/methodology/approach – The method used was a descriptive, quantitative research, applied to 1032 individuals residing in Brazil and Portugal, analyzed using Structural Equation Modeling. Findings – Holistic Helixes of Innovation strongly influence Eco-innovation. This finding can contribute to the promotion of public policies to encourage integration among stakeholders of holistic innovation helices, such as universities, government, industries, technology parks, spin-offs, incubators, startup, consulting teams, non-governmental organizations, shareholders, suppliers, and customers. The study also shows the positive influence of eco-innovation on environmental practices, cleaner production, social actions, smart cities, sustainable development, with emphasis on regional development. Research, Practical & Social implications – The Eco-innovation precepts are key to trigger positive influences on socio-environmental aspects, smart cities and regional and sustainable development. In this sense, organizations and governments can contribute to society, with greater efficiency, allocating resources in projects that develop socio-environmental innovations. Originality/value – It is relevant for science to know the variables that can help governments and other organizations to develop policies and actions to promote the improvement of people's quality of life from a long-term triple bottom line perspective.
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